Comparison of Different Immunosorbent Assays for Citrus Tristeza Virus (CTV) Using CTV Specific Monoclonal and Polyclonal Antibodies
نویسندگان
چکیده
منابع مشابه
An asymmetric PCR-ELISA typing assay for Citrus tristeza virus (CTV)
Typing of CTV based on coat protein (CP) gene has been approached in diverse ways. The basis for the assay focused on in this paper was presented some years ago and has been subject to several improvements since its appearance. Although the results obtained with this assay have been presented in diverse scientiic meetings, theses and technical seminars, so far no paper has explained the rationa...
متن کاملCitrus tristeza virus (CTV) Causing Proteomic and Enzymatic Changes in Sweet Orange Variety “Westin”
Citrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as ...
متن کاملProduction and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis
Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and...
متن کاملMonoclonal and Polyclonal Antibodies Specific to Human Fibromodulin
Background: The unique expression of fibromodulin (FMOD) in patients with chronic lymphocytic leukemia (CLL) has been previously reported. Detecting FMOD in CLL patients using specific anti-FMOD mAbs might provide a promising method in detection, monitoring, and prognosis of CLL. Objectives: In this study, we aimed for producing specific antibodies agains...
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ژورنال
عنوان ژورنال: International Organization of Citrus Virologists Conference Proceedings (1957-2010)
سال: 1991
ISSN: 0074-7203,2313-5123
DOI: 10.5070/c53sb6f7wq